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Product Description
Human Putative heat shock protein HSP 90-alpha A4 (HSP90AA4P) ELISA Kit | AE60829HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: HSP90AA4P
Alternative Name: HSP90Ad; HSPCAL2;
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.068 ng/mL
Intra-Assay: ≤6.9%
Inter-Assay: ≤8.7%
Recovery: 0, 88
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate HSP90AA4P in samples. An antibody specific for HSP90AA4P has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSP90AA4P present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP90AA4P is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP90AA4P bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: N/A
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
