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Product Description
Mouse ATP-binding cassette sub-family A member 9 (ABCA9) ELISA Kit | AE23087MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: ABCA9
Alternative Name: DKFZp686F2450; EST640918; MGC75415; ATP-binding cassette A9|ATP-binding cassette; sub-family A; member 9
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.058 ng/mL
Intra-Assay: ≤5.4%
Inter-Assay: ≤8.2%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ABCA9 in samples. An antibody specific for ABCA9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: ATP-binding cassette sub-family A member 9 is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White) . This gene is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of this gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import.Widely expressed with higher expression in heart.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
