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Product Description
Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) ELISA Kit | AE27390MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: PIN1
Alternative Name: DOD; UBL5; peptidyl-prolyl cis/trans isomerase; NIMA-interacting|prolyl isomerase|protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1|protein (peptidylprolyl cis/trans isomerase) NIMA-i
Application: ELISA
Range: 1.56-100 pg/mL
Sensitivity: 0.39 pg/mL
Intra-Assay: ≤4.2%
Inter-Assay: ≤8.1%
Recovery: 0, 95
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PIN1 in samples. An antibody specific for PIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8), such as PIN1, catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. PIN1 is the only PPIase that specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. PIN1-catalyzed conformational regulation has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, germ cell development, neuronal differentiation, and survival. Both Drosophila and human DOD contain a WW domain for protein-protein interactions and a peptidylprolyl cis-trans isomerase (PPIase) domain, and they are related to the Ess1 cell division gene of Saccharomyces cerevisiae.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
