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Product Description
Mouse Peroxiredoxin-1 (PRDX1) ELISA Kit | AE60050MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: PRDX1
Alternative Name: MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer-enhancing factor A|proliferation-associated gene A|thioredoxin-dependent peroxide reductase 2
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.3%
Inter-Assay: ≤7.1%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRDX1 in samples. An antibody specific for PRDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Peroxiredoxin-1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8 (+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
