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Product Description
Mouse PR domain zinc finger protein 8 (PRDM8) ELISA Kit | AE26094MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: PRDM8
Alternative Name: PFM5; PR-domain containing protein 8
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤5.4%
Inter-Assay: ≤10.2%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRDM8 in samples. An antibody specific for PRDM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities.In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
