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Product Description
Mouse Pulmonary Surfactant-associated protein C (SFTPC) ELISA Kit | AE19824MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: SFTPC
Alternative Name: PSP-C; SFTP2; SMDP2; SP-C; pulmonary surfactant apoprotein-2 SP-C|surfactant; pulmonary-associated protein C
Application: ELISA
Range: 0.625-40 ng/mL
Sensitivity: 0.241 ng/mL
Intra-Assay: ≤5.7%
Inter-Assay: ≤6.9%
Recovery: 0, 99
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SFTPC in samples. An antibody specific for SFTPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: SP-C is a fragment of a much larger precursor protein of 21 kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.Pulmonary surfactant-associated protein C is a membrane protein which manufactures surfactant.Humans and animals born lacking SP-C tend to develop progressive interstitial pneumonitis.The SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
