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Product Description
Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit | AE12503MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: UBE2I
Alternative Name: C358B7.1; P18; UBC9; OTTHUMP00000198126|OTTHUMP00000198127|SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzym
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.125 ng/mL
Intra-Assay: ≤6.1%
Inter-Assay: ≤11.5%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate UBE2I in samples. An antibody specific for UBE2I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2I bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.The protein has a predicted sequence of 158 amino acids and can support the growth of yeast ubc9 temperature-sensitive mutants at non-permissive temperatures, indicating that their gene is a functional homolog of yeast ubc9.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
