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Product Description
Mouse Zinc transporter 7 (SLC30A7) ELISA Kit | AE18735MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: SLC30A7
Alternative Name: DKFZp686M0368; ZNT7; ZnT-7; ZnTL2; zinc transporter ZnT-7|zinc transporter like 2
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.053 ng/mL
Intra-Assay: ≤3.4%
Inter-Assay: ≤7.9%
Recovery: 0, 99
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC30A7 in samples. An antibody specific for SLC30A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT) /SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc.The deduced proteins share 96% amino acid identity. Mouse Znt7 contains 6 transmembrane domains, a histidine-rich loop between transmembrane domains 4 and 5, cytoplasmic N and C termini, several phosphorylation sites, and an N-glycosylation site. Northern blot analysis of mouse tissues detected highest expression in liver, spleen, and small intestine. In rat epithelial and human fibroblast cell lines, ZNT7 clustered at the perinuclear region and showed punctate staining in the cytoplasm, indicative of cytoplasmic vesicle localization.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
