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Product Description
Rabbit Bile salt export pump (ABCB11) ELISA Kit | AE23095RB | Abebio
Species Reactivity: Rabbit (Oryctolagus cuniculus)
Abbreviation: ABCB11
Alternative Name: ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP; ABC member 16; MDR/TAP subfamily|bile salt export pump|progressive familial intrahepatic cholestasis 2|sister p-glycoprotein
Application: ELISA
Range: 31.25-2000 pg/mL
Sensitivity: 14.8 pg/mL
Intra-Assay: ≤5.3%
Inter-Assay: ≤7.9%
Recovery: 0, 87
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ABCB11 in samples. An antibody specific for ABCB11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: ABCB11 is an ABC transporter named BSEP (Bile Salt Export Pump), or sPgp (sister of P-glycoprotein) . This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) .This protein is a member of the MDR/TAP subfamily. Some members of the MDR/TAP subfamily are involved in multidrug resistance. This particular protein is responsible for the transport of taurocholate and other cholate conjugates from hepatocytes (liver cells) to the bile. In humans, the activity of this transporter is the major determinant of bile formation and bile flow.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
