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Product Description
Rabbit Pyruvate kinase isozymes M1/M2 (PKM2) ELISA Kit | AE27267RB | Abebio
Species Reactivity: Rabbit (Oryctolagus cuniculus)
Abbreviation: PKM2
Alternative Name: CTHBP; MGC3932; OIP3; PK3; PKM; TCB; THBP1; OPA-interacting protein 3|PK; muscle type|thyroid hormone-binding protein; cytosolic
Application: ELISA
Range: 78.12-5000 pg/mL
Sensitivity: 33 pg/mL
Intra-Assay: ≤6.6%
Inter-Assay: ≤8.2%
Recovery: 1
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PKM2 in samples. An antibody specific for PKM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Pyruvate Kinase M2 (M2-PK) is strongly overexpressed in a metabolic state specific for a variety of tumor cells. Recent reports indicate that Tumor M2-PK is a marker for renal cell carcinoma and. lung cancer. It has also been investigated in. pancreatic cancer and gastric cancer with promising results. M2-PK is encoded by the gene PKM2 which does also encode for the pyruvate kinase isoenzyme type M1 (PKM1 or M1-PK) . The M-gene consists of 12 exons and 11 introns. PKM1 and PKM2 are different splicing products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23 amino acids within a 56 amino acid stretch (aa 378-434) at their carboxy terminus.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
