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Product Description
Rat 6-phosphofructokinase, muscle type (PFKM) ELISA Kit | AE27886RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PFKM
Alternative Name: GSD7; MGC8699; PFK-1; PFK1; PFKA; PFKX; 6-phosphofructo-1-kinase|phosphofructo-1-kinase isozyme A|phosphofructokinase; polypeptide X|phosphohexokinase
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.112 ng/mL
Intra-Assay: ≤5.9%
Inter-Assay: ≤7.2%
Recovery: 0, 89
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PFKM in samples. An antibody specific for PFKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Phosphofructokinase-1 (PFK-1) is the most important regulatory enzyme (EC 2.7.1.11) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. PFK-1 catalyzes one of the important "committed" steps of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1, 6-bisphosphate and ADP. PFK1 is allosterically inhibited by ATP and citrate (from the citric acid cycle) and its product. It is also inhibited by low pH to prevent the accumulation of hydrogen ions in muscle. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2, 6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
