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Product Description
Rat Activity-dependent neuroprotector homeobox protein (ADNP) ELISA Kit | AE24008RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: ADNP
Alternative Name: ADNP1; KIAA0784; ADNP homeobox 1|OTTHUMP00000031275|OTTHUMP00000165329|activity-dependent neuroprotective protein|activity-dependent neuroprotector
Application: ELISA
Range: 31.25-2000 pg/mL
Sensitivity: 16 pg/mL
Intra-Assay: ≤6.2%
Inter-Assay: ≤8.5%
Recovery: 1, 03
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ADNP in samples. An antibody specific for ADNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADNP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. Activity Dependent Neuroprotector contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
