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Product Description
Rat Coagulation factor IX (F9) ELISA Kit | AE61132RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: F9
Alternative Name: RP6-88D7.1; FIX; HEMB; MGC129641; MGC129642; PTC; Christmas factor|factor 9|plasma thromboplastic component
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.067 ng/mL
Intra-Assay: ≤6.4%
Inter-Assay: ≤7.9%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate F9 in samples. An antibody specific for F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Factor IX is one of the serine proteases (EC 3.4.21.22) of the coagulation system; it belongs to peptidase family S2. Deficiency of this protein causes hemophilia B. It was discovered after a young boy named Stephen Christmas was found to be lacking this⃀Ϯ⁰Ϯ
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
