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Product Description
Rat D-Lactate Dehydrogenase (D-LDH) ELISA Kit | AE62851RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: D-LDH
Alternative Name: N/A
Application: ELISA
Range: 0.78-50 ng/mL
Sensitivity: 0.35 ng/mL
Intra-Assay: ≤5.3%
Inter-Assay: ≤6.9%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate D-LDH in samples. An antibody specific for D-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyD-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for D-LDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of D-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes with each acting on either D-lactate (EC 1.1.2.4) or L-lactate (EC 1.1.2.3) . The other two are NAD (P) - dependent enzymes with each acting on either D-lactate (EC 1.1.1.28) or L-lactate (EC 1.1.1.27) . This article is about the NAD (P) - dependent L-lactate dehydrogenase. The five isozymes that are usually described in the literature each contain four subunits. The major isozymes of skeletal muscle and liver, M4, has four muscle (M) subunits; while H4 is the main isozymes for heart muscle in most species, containing four heart (H) subunits. The other variants contain both types of subunits.Usually LDH-2 is the predominant form in the serum. A LDH-1 level higher than the LDH-2 level, suggests myocardial infarction. The use of this phenomenon to diagnose infarction has been largely superseded by the use of Troponin I or T measurement.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
