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Product Description
Rat Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit | AE18163RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: SMAD7
Alternative Name: CRCS3; FLJ16482; MADH7; MADH8; MAD (mothers against decapentaplegic; Drosophila) homolog 7|MAD; mothers against decapentaplegic homolog 7|Mothers against decapentaplegic; drosophila; homolog of; 7|S
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.055 ng/mL
Intra-Assay: ≤6.3%
Inter-Assay: ≤11.3%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SMAD7 in samples. An antibody specific for SMAD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: MAD proteins, originally defined in Drosophila, are essential components of the signaling pathways of the transforming growth factor-beta receptor family. Using a differential display approach in cultured endothelial cells subjected to multiple soluble and biomechanical stimuli, Topper et al. (1997) isolated a human endothelial cell cDNA encoding MADH7, which they called SMAD7. The predicted 426-amino acid MADH7 protein lacks the C-terminal putative phosphorylation sites present in other MAD proteins, suggesting that it may be distinctly regulated. In situ hybridization and immunohistochemical analyses on human tissues showed that MADH7 is expressed predominantly in vascular endothelium.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
