Product Description
Rat Muscarinic acetylcholine receptor M2 (CHRM2) ELISA Kit | AE61439RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: CHRM2
Alternative Name: FLJ43243; HM2; MGC120006; MGC120007; 7TM receptor|OTTHUMP00000208394|OTTHUMP00000208395|cholinergic receptor; muscarinic 2; isoform a|muscarinic M2 receptor|muscarinic acetylcholine receptor M2
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.053 ng/mL
Intra-Assay: ≤3.8%
Inter-Assay: ≤8.1%
Recovery: 1, 03
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate CHRM2 in samples. An antibody specific for CHRM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: He is a muscarinic acetylcholine receptor.M2 muscarinic receptors act via a Gi type receptor, which causes a decrease in cAMP in the cell, generally leading to inhibitory-type effects.In addition, they modulate muscarinic potassium channels. In the heart, this contributes to a decreased heart rate.The M2 muscarinic receptors are located in the heart, where they act to slow the heart rate down to normal sinus rhythm after stimulatory actions of the sympathetic nervous system, by slowing the speed of depolarization. They also reduce contractile forces of the atrial cardiac muscle, and reduce conduction velocity of the atrioventricular node (AV node) . However, they have no effect on the contractile forces of the ventricular muscle.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .