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Product Description
Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit | AE28917RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: P2RY14
Alternative Name: GPR105; KIAA0001; P2Y14; G protein coupled receptor for UDP-glucose|G protein-coupled receptor 105|P2Y purinoceptor 14|P2Y (14) receptor|P2Y14 receptor
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.053 ng/mL
Intra-Assay: ≤6.1%
Inter-Assay: ≤11.4%
Recovery: 1, 09
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate P2RY14 in samples. An antibody specific for P2RY14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP2RY14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P2RY14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: P2Y purinoceptor 14 is a protein belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.The predicted 338-amino acid protein contains transmembrane domains and a tyrosine kinase phosphorylation site.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
