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Product Description
Rat Pentosidine (PTD) ELISA Kit | AE28010RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PTD
Alternative Name: N/A
Application: ELISA
Range: 15.6-1000 pmol/mL
Sensitivity: 3.9pmol/mL
Intra-Assay: ≤5.1%
Inter-Assay: ≤8.9%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PTD in samples. An antibody specific for PTD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Pentosidine is a well characterized and easily detected member of this large class of compounds. AGEs are biochemicals formed continuously under normal circumstances, but more rapidly under a variety of stresses, especially oxidative stress and hyperglycemia. They serve as markers of stress and act as toxins themselves. Pentosidine is typical of the class, except that it fluoresces, which allows it to be seen and measured easily. Because it is well characterized, it is often studied to provide new insight into the biochemistry of AGE compounds in general. Derived from ribose, a pentose, pentosidine forms fluorescent cross-links between the arginine and lysine residues in collagen. It is formed in a reaction of the amino acids with the Maillard reaction products of ribose. In patients with Diabetes mellitus type 2, pentosidine correlates with the presence and severity of diabetic complications.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
