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Product Description
Rat Proenkephalin-B (PDYN) ELISA Kit | AE28094RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PDYN
Alternative Name: MGC26418; PENKB; beta-neoendorphin|beta-neoendorphin-dynorphin|dynorphin|leu-enkephalin|leumorphin|preprodynorphin|preproenkephalin B|rimorphin
Application: ELISA
Range: 15.6-1000 pg/mL
Sensitivity: 3.9 pg/mL
Intra-Assay: ≤4.2%
Inter-Assay: ≤6.9%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PDYN in samples. An antibody specific for PDYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Prodynorphin is a opioid polypeptide hormone involved with chemical signal transduction and cell communication. The gene for prodynorphin is expressed in the endometrium and the striatum, and its gene map locus is 20pter-p12. Prodynorphin is a basic building block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds, and which are also critical in learning and memory. The prodynorphin enzyme is identical in humans and chimps, but the regulatory promoter sequences have been shown to exhibit marked differences.The gene is thought to influence perception, as well as susceptibility to drug dependence, and is expressed more readily in human beings than in other primates.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
