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Product Description
Rat Ras GTPase-activating protein SynGAP (SYNGAP1) ELISA Kit | AE16075RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: SYNGAP1
Alternative Name: DASS-97D12.6; DKFZp761G1421; KIAA1938; MRD5; RASA1; RASA5; SYNGAP; Ras GTPase-activating protein SynGAP|neuronal RasGAP|synaptic Ras GTPase activating protein 1|synaptic Ras GTPase activating protei
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.7%
Inter-Assay: ≤7.1%
Recovery: 1, 03
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SYNGAP1 in samples. An antibody specific for SYNGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: SYNGAP1 is a ras GTPase-activating protein that is critical for cognition and synapse function. Mutations in humans cause mental retardation. Several mutations in the SYNGAP1 gene were identified as the cause of mental retardation. Mental retardation is sometimes associated with syndromes of other defects caused by the same gene, but SYNGAP1-associated mental retardation is not; it is therefore called non-syndromic mental retardation. Since neither of the parents of children with this condition have the mutation, this means it was a sporadic mutation that occurred during division of the parents' gametes (meiosis) or fertilization of the egg. It is a dominant mutation, which means that the individual will be retarded if only one allele is mutated.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
