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Product Description
Rat Vanillylmandelic acid (VMA) ELISA Kit | AE63311RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: VMA
Alternative Name: Vanillyl Mandelic Acid
Application: ELISA
Range: 123.5-10000 ng/mL
Sensitivity: 43.7 ng/mL
Intra-Assay: ≤4.5%
Inter-Assay: ≤6.5%
Recovery: 0, 84
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate VMA in samples. An antibody specific for VMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Moderately elevated VMA (vanillylmandelic acid) can be caused by a variety of factors such as essential hypertension, intense anxiety, intense physical exercise, and numerous drug interactions (including some over-the-counter medications and herbal products) . Medications that may interfere with catecholamines and their metabolites include amphetamines and amphetamine-like compounds, appetite suppressants, bromocriptine, buspirone, caffeine, chlorpromazine, clonidine, disulfiram, diuretics (in doses sufficient to deplete sodium), epinephrine, glucagon, guanethidine, histamine, hydrazine derivatives, imipramine, levodopa, lithium, MAO inhibitors, melatonin, methyldopa, morphine, nitroglycerin, nose drops, propafenone, radiographic agents, rauwolfia alkaloids (Reserpine), tricyclic antidepressants, and vasodilators. The effects of some drugs on catecholamine metabolite results may not be predictable.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
