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Product Description
Bovine Protein phosphatase 1A (PPM1A) ELISA Kit | AE26404BO | Abebio
Species Reactivity: Bovine (Bos taurus; Cattle)
Abbreviation: PPM1A
Alternative Name: FLJ42306; MGC9201; PP2C-ALPHA; PP2CA; PP2Calpha; phosphatase 2C alpha|protein phosphatase 1A|protein phosphatase 2C alpha isoform
Application: ELISA
Range: 31.25-2000 pg/mL
Sensitivity: 14.0 pg/mL
Intra-Assay: ≤5.3%
Inter-Assay: ≤9.8%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PPM1A in samples. An antibody specific for PPM1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPM1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPM1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPM1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
