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Product Description
Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit | AE23796HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: ADAM28
Alternative Name: ADAM23; MDC-Lm; MDC-Ls; MDCL; eMDCII; a disintegrin and metalloproteinase domain 28|metalloproteinase-like; disintegrin-like; and cysteine-rich protein-L
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.059 ng/mL
Intra-Assay: ≤3.2%
Inter-Assay: ≤7.2%
Recovery: 0, 87
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ADAM28 in samples. An antibody specific for ADAM28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: ADAM28 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
