Product Description
Human Osteonectin (ON) ELISA Kit | AE15002HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: SPOCK2
Alternative Name: FLJ97039; testican-2;
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤3.8%
Inter-Assay: ≤8.3%
Recovery: 0, 99
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. Some current research shows a correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis. Osteonectin is an acidic, secreted extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. Additional functions of osteonectin beneficial to tumor cells include angiogenesis, proliferation and migration.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .