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Product Description
Human Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit | AE28231HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PDGF-BB
Alternative Name: N/A
Application: ELISA
Range: 62.5-4000 pg/mL
Sensitivity: 25.9 pg/mL
Intra-Assay: ≤4.6%
Inter-Assay: ≤7.7%
Recovery: 0, 87
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PDGF-BB in samples. An antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGF-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-BB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The Platelet-Derived Growth Factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, -DD) and one heterodimer (PDGF-AB) . These proteins and the related VEGF family proteinsshare the conserved PDGF/VEGF homology domain characterized by a pattern of highly conserved cysteine residues, which form the cysteine knot motif. PDGF-B is synthesized as a pre-pro-protein, which has a signal peptide, a mature region, an N- terminal pro-peptide, and a C-terminal extension. The C-terminal extension contains a conserved cell retention motif that confers retention of the secreted PDGF-BB within the pericellular space. Most cells produce both PDGF-A and B chains. The individual chains are assembled stochastically into disulfide-linked inactive homodimeric or heterodimeric precursors in the endoplasmic reticulum.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
