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Product Description
Human Pro-gastrin-releasing peptide (ProGRP) ELISA Kit | AE25789HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: ProGRP
Alternative Name: Pro-GRP
Application: ELISA
Range: 12.35-1000 pg/mL
Sensitivity: 4.95 pg/mL
Intra-Assay: ≤4.6%
Inter-Assay: ≤8.3%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ProGRP in samples. An antibody specific for ProGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyProGRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ProGRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ProGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Pro-gastrin-releasing peptide (ProGRP) was the new prognostic marker of lung cancer. PreproGRP begins with signal peptidase cleavage to generate the proGRP, which is then processed (by proteolytic cleavages), to form smaller GRP peptides. PreproGRP begins with signal peptidase cleavage to generate the proGRP, which is then processed (by proteolytic cleavages), to form smaller GRP peptides. These smaller peptides are released by the post-ganglionic fibers of the vagus nerve which innervate the G cells of the stomach and stimulate them to release gastrin. GRP regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
