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Product Description
Human Probable histone-lysine N-methyltransferase NSD2 (WHSC1) ELISA Kit | AE11200HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: WHSC1
Alternative Name: RP11-262P20.3; FLJ23286; KIAA1090; MGC176638; MMSET; NSD2; REIIBP; TRX5; WHS; IL5 promoter REII region-binding protein|OTTHUMP00000196943|Wolf-Hirschhorn syndrome candidate 1 protein|multiple myelom
Application: ELISA
Range: 0.31-20 ng/mL
Sensitivity: 0.156 ng/mL
Intra-Assay: ≤5.2%
Inter-Assay: ≤9.6%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate WHSC1 in samples. An antibody specific for WHSC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWHSC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WHSC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WHSC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: WHSC1 encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t (4;14) (p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
