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Product Description
Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit | AE28275HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PDCD6IP
Alternative Name: AIP1; Alix; DRIP4; HP95; MGC17003; ALG-2 interacting protein 1|ALG-2 interacting protein X|apoptosis-linked gene 2-interacting protein X|dopamine receptor interacting protein 4|programmed cell death
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.115 ng/mL
Intra-Assay: ≤5.3%
Inter-Assay: ≤9.2%
Recovery: 0, 85
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PDCD6IP in samples. An antibody specific for PDCD6IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD6IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD6IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD6IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Programmed cell death 6-interacting protein is a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
