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Product Description
Human Protein Induced Vitamin K Absence or Antagonist-II (PIVKA-II) ELISA Kit | AE27315HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PIVKA-II
Alternative Name: N/A
Application: ELISA
Range: 0.31-20 ng/mL
Sensitivity: 0.156 ng/mL
Intra-Assay: ≤3.5%
Inter-Assay: ≤9.1%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PIVKA-II in samples. An antibody specific for PIVKA-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIVKA-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIVKA-II is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIVKA-II bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: protein induced by vitamin K absence/antagonist-II (PIVKA-II), is an abnormal form of the coagulation protein, prothrombin. Normally, the prothrombin precursor undergoes post-translational carboxylation (addition of a carboxylic acid group) by gamma-glutamyl carboxylase in the liver prior to secretion into plasma. DCP/PIVKA-II may be detected in people with deficiency of vitamin K (due to poor nutrition or malabsorption) and in those taking warfarin or other medication that inhibits the action of vitamin K.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
