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Product Description
Human Protein tyrosine phosphatase 1B (PTP1B) ELISA Kit | AE25122HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PTPN1
Alternative Name: PTP1B; protein tyrosine phosphatase 1B|protein tyrosine phosphatase; placental
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.054 ng/mL
Intra-Assay: ≤6.0%
Inter-Assay: ≤10.4%
Recovery: 0, 9
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PTPN1 in samples. An antibody specific for PTPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Tyrosine-protein phosphatase non-receptor type 1 is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
