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Product Description
Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PPP3CB) ELISA Kit | AE26235HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PPP3CB
Alternative Name: RP11-345K20.1; CALNA2; CALNB; CNA2; CAM-PRP catalytic subunit|calcineurin A beta|calcineurin A2|calmodulin-dependent calcineurin A subunit beta|protein phosphatase 3; catalytic subunit; beta|protein
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.064 ng/mL
Intra-Assay: ≤5.8%
Inter-Assay: ≤10.8%
Recovery: 0, 89
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PPP3CB in samples. An antibody specific for PPP3CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP3CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP3CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca (2+) -binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B (PPP3R1) in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha (PPP3CA) and beta (PPP3CB), of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma (PPP3CC), is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
