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Product Description
Human Sex hormone-binding globulin (SHBG) ELISA Kit | AE19634HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: SHBG
Alternative Name: ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein|sex steroid-binding protein|testis-specific androgen-binding protein|testosterone-binding beta-globulin|testosterone-estradiol-binding
Application: ELISA
Range: 1.56-100 ng/mL
Sensitivity: 0.62 ng/mL
Intra-Assay: ≤4.3%
Inter-Assay: ≤7.5%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SHBG in samples. An antibody specific for SHBG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySHBG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHBG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHBG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: SHBG is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. These sex hormones circulate in the bloodstream, bound mostly to SHBG and to some degree bound to serum albumin. Only a small fraction is unbound, or "free, " and thus biologically active and able to enter a cell and activate its receptor. The SHBG inhibits the function of these hormones. Thus bioavailability of sex hormones is influenced by the level of SHBG. SHBG is produced by the liver cells and is released into the bloodstream. Other sites that produce SHBG are the brain, uterus, and placenta and vagina. In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
