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Product Description
Human Spermidine synthase (SRM) ELISA Kit | AE15196HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: SRM
Alternative Name: PAPT; SPDSY; SPS1; SRML1; putrescine aminopropyltransferase|spermidine synthase-1
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.2%
Inter-Assay: ≤7.8%
Recovery: 1, 06
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SRM in samples. An antibody specific for SRM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: It has SH2, SH2', and SH3 domains and a tyrosine residue for autophosphorylation in the kinase domain but lacks an N-terminal glycine for myristylation and a C-terminal tyrosine which, when phosphorylated, suppresses kinase activity. These are structural features of the recently identified Tec family of nonreceptor tyrosine kinases. The Srm N-terminal unique domain, however, lacks the structural characteristics of the Tec family kinases, and the sequence similarity is highest to Src in the SH region. Mutant mice were generated by gene targeting in embryonic stem cells but displayed no apparent phenotype as in mutant mice expressing Src family kinases. These results suggest that Srm constitutes a new family of nonreceptor tyrosine kinases that may be redundant in function.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
