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Product Description
Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit | AE13213HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: TRPV1
Alternative Name: DKFZp434K0220; VR1; capsaicin receptor|transient receptor potential vanilloid 1a|transient receptor potential vanilloid 1b|vanilloid receptor subtype 1
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.114 ng/mL
Intra-Assay: ≤4.6%
Inter-Assay: ≤8.3%
Recovery: 1, 04
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate TRPV1 in samples. An antibody specific for TRPV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: TRPV1 is a nonselective cation channel that may be activated by a wide variety of exogenous and endogenous physical and chemical stimuli. The best-known activators of TRPV1 are heat greater than 43°C and capsaicin, the pungent compound in hot chili peppers. The activation of TRPV1 leads to painful, burning sensation. Its endogenous activators include: low pH, the endocannabinoid anandamide, N-arachidonoyl-dopamine. TRPV1 receptors are found mainly in the nociceptive neurons of the peripheral nervous system, but they have also been described in many other tissues, including the central nervous system. TRPV1 is involved in the transmission and modulation of pain, as well as the integration of diverse painful stimuli.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
