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Product Description
Pig Somatostatin (SS) ELISA Kit | AE15342PI | Abebio
Species Reactivity: Pig (Sus scrofa; Porcine)
Abbreviation: SST
Alternative Name: SMST; somatostatin-14|somatostatin-28
Application: ELISA
Range: 6.17-500 pg/mL
Sensitivity: 2.74 pg/mL
Intra-Assay: ≤4.6%
Inter-Assay: ≤9.4%
Recovery: 1, 02
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SST in samples. An antibody specific for SST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G-protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones.Somatostatin was first discovered in hypothalamic extracts and identified as a hormone that inhibited secretion of growth hormone. Subsequently, somatostatin was found to be secreted by a broad range of tissues, including pancreas, intestinal tract and regions of the central nervous system outside the hypothalamus. Two forms of somatostatin are synthesized. They are referred to as SS-14 and SS-28, reflecting their amino acid chain length. Both forms of somatostatin are generated by proteolytic cleavage of prosomatostatin, which itself is derived from preprosomatostatin.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
