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Product Description
Chicken anti-Rabbit IgG (H&L), HRP conjugated | AS10 833 | Agrisera
Immunogen: Purified Rabbit IgG
Host: Chicken
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified chicken IgY.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated, antibodies
Recommended Dilutions: The optimal working dilution should be determined by the investigator
Molecular weight: N/A
Confirmed Reactivity: Rabbit IgG (H&L)
Predicted Reactivity: Rabbit IgG (H&L)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Background: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: HRP (horse radish peroxidase)
Research Area: Immunoassays
