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Product Description
Chicken anti-Rat IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to human or rabbit IgG or serum proteins | AS12 1973 | Agrisera
Immunogen: Purified rat IgG
Host: Chicken
Conjugation: DyLight® 550
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified chicken IgG.
Format: Lyophilized
Tested Application: none
Related Products: other dylight anti-rat secondary antibodies
Recommended Dilutions: 1 : 20-1 : 2000 for most applications
Molecular weight: N/A
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from human or rabbit serum proteins from human or rabbit.
Background: Chicken anti-Rat IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to DyLight® 550, which binds to all rat IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L) .DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Reconstitution: For reconstitution add 0, 55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0, 5 mg of antibody in 0, 55 ml of sterile water add 0, 55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: DyLight® 550
Research Area: Immunoassays
