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Product Description
Donkey anti-Mouse IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG | AS12 1935 | Agrisera
Immunogen: Purified Mouse IgG, whole molecule
Host: Donkey
Conjugation: DyLight® 550
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified donkey IgG.
Format: Lyophilized
Tested Application: N/A
Related Products: other dylight anti-mouse secondary antibodies
Recommended Dilutions: 1 : 20-1 : 2000 for most applications
Molecular weight: N/A
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Background: Donkey anti-Mouse IgG (H&L) - DyLight® 550 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight® 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L) .DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C, Product is stable for 4 weeks at 2-8°C after rehydration, For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
TAIR Nnumbre: N/A
Category: DyLight® 550
Research Area: Immunoassays
