Product Description
Donkey anti-Rabbit IgG (H&L), ALP conjugated | AS10 841 | Agrisera
Immunogen: Purified Rabbit IgG
Host: Donkey
Conjugation: ALP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified donkey IgG.
Format: Liquid
Tested Application: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS09 607 | Goat anti-rabbit IgG (H&L) ALP (alkaline phosphatase) conjugated
Recommended Dilutions: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), , 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Rabbit IgG (H&L)
Predicted Reactivity: Rabbit IgG (H&L)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Background: Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Reconstitution: N/A
Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: ALP (alkaline phosphatase)
Research Area: Immunoassays