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Product Description
FtsH2 | FtsH2 positive control/quantitation standard | AS11 1789S | Agrisera
Immunogen: N/A
Host: N/A
Conjugation: N/A
Clonality: N/A
Isotype: N/A
Purity: N/A
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS11 1789 | anti-FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11Collection of other protein standardsCollection of global antibodiesCollection of antibodies to photosynthetic proteins Plant protein extraction buffer
Recommended Dilutions: Standard curve: 3 loads are recommended (0.5, 2 and 4µl) .For most applications a sample load of 0.2µg of chlorophyll will give a FtsH2 signal in this range.Positive control: load per well: a 2 µl load is optimal for most chemiluminescent detection systems.Standard curve: 3 loads are recommended (0.5, 2 and 4µl) .For most applications a sample load of 0.2 µg of chlorophyll will give a FtsH2 signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems.Non-disulphie dependent dimers and complexes can be also detected using standard western blot methods withmore sensitive detection reagents as ECL Advance or West Pico when loading per well more standard than recommended. They have not been included in the standard calibration.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Molecular weight: 75 kDa
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: Concentration: after adding 225 µl of sterile milliQ water final concentration of the standard is 0.1 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra careto mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing,
Background: FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Reconstitution: For reconstitution add 225 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Protein Standards-Quantitation
Research Area: Plant Biology
