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Product Description
Goat anti-Chicken IgY (H&L), HRP conjugated | AS09 603 | Agrisera
Immunogen: Purified chicken IgY, whole molecule,
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS10 1489 | Rabbit anti-Chicken IgY (H&L), HRP conjugated, secondary antibodiesAS09 606 | Goat anti-hen IgY (H&L) AP conjugated, secondary antibodiesAS09 610 | Goat anti-hen IgY (H&L) biotin conjugated, secondary antibodies
Recommended Dilutions: 1 : 10 000 -1 : 150 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 20 000 and 1 : 10 000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Chicken IgY heavy and light chains (H&L) .
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
Background: Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: HRP (horse radish peroxidase)
Research Area: Immunoassays
