Product Description
Goat anti-Human IgG (H&L), HRP conjugated | AS10 764 | Agrisera
Immunogen: Purified human IgG, whole molecule
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: Secondary antibodies to human immunoglobulins
Recommended Dilutions: The optimal working dilution should be determined by the investigator
Molecular weight: N/A
Confirmed Reactivity: Heavy chains on human IgG and light chanins on all human immunoglobulins
Predicted Reactivity: N/A
Not reactive in: Non immunoglobulin human serum proteins
Additional Information: N/A
Background: Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: N/A
Research Area: Immunoassays, Immunology