Product Description
Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, horse, human, pig or rabbit serum | AS10 1427 | Agrisera
Immunogen: Purified mouse IgG (H&L) AAA51107
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: other HRP-conjugated secondary antibodies against mouse IgG (H&L)
Recommended Dilutions: 1: 2000 - 1: 5000 (ELISA, WB), 1: 500 - 1: 5000 (IHC)
Molecular weight: N/A
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L) .
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: HRP (horse radish peroxidase)
Research Area: Immunoassays