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Product Description
Goat anti-Rabbit IgG (H&L), DyLight® 350 conjugated | AS11 1812 | Agrisera
Immunogen: Purified Rabbit IgG, whole molecule
Host: Goat
Conjugation: DyLight® 350
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
Related Products: other anti-rabbit DyLight® conjugated secondary antibodies Plant and algal protein extraction buffer
Recommended Dilutions: 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
Molecular weight: N/A
Confirmed Reactivity: Rabbit IgG heavy and light chains (H&L)
Predicted Reactivity: Rabbit IgG Heavy and Light chains (H&L)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
Background: Goat anti-rabbit IgG, DyLight® 350 Conjugate is a secondary antibody conjugated toDyLight® 350, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: DyLight® 350
Research Area: Immunoassays
