Product Description
Goat anti-Rat IgG (H&L), ALP conjugated, min, reactivity to Human and mouse IgG, highly adsorbed against mouse IgG | AS10 663 | Agrisera
Immunogen: Purified Rat IgG, whole molecule
Host: Goat
Conjugation: ALP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Liquid
Tested Application: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Related Products: N/A
Recommended Dilutions: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Heavy chains on Rat IgG and with the light chains on all Rat immunoglobulins based on IEP
Predicted Reactivity: Heavy chains on Rat IgG and with the Light chains on all Rat immunoglobulins based on IEP
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: This antibody is highly cross absorbed against mouse IgG, No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis, No reactivity is observed to human or mouse IgG based on immunoelecrophoresis,
Background: Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Reconstitution: N/A
Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: ALP (alkaline phosphatase)
Research Area: Immunoassays