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Product Description
GN | Gnom | AS16 3980 | Agrisera
Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana GN protein sequence, UniProt: Q42510, TAIR: At1g13980
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: collection of antibodies to proteins involved in plant development
Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 162 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Nitrocellulose membrane with pore size 0.45 µm is recommended as well as TBS-T as antibody incubation buffer. This antibody is also recognzing recombinant GN fused to GFP.
Background: GN (Gnom) activates the ARF proteins by exchanging bound GDP for free GTP and plays a role in vesicular protein sorting. Acts as the major regulator of endosomal vesicle trafficking but is also involved in the endocytosis process. GN is required for correct cell wall organization leading to normal cell adhesion during seedling development amd plays also an essential role in hydrotropism of seedling roots. Alternative names: Pattern formation protein EMB30, Protein EMBRYO DEFECTIVE 30, Protein MIZU-KUSSEI2, Protein VASCULAR NETWORK 7
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: At1g13980
Category: Signal transduction
Research Area: Cell Signaling
