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Product Description
BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic) | AS19 4321 | Agrisera
Immunogen: KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS05 073 | Anti-CAH3 | Carbonic anhydrase, rabbit antibodiesAS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodiesAS17 4114 | Anti-Gamma CA | Gamma Carbonic anhydrases
Recommended Dilutions: 1 : 20 000 (WB)
Molecular weight: 37, 5 | 25, 3 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Extraction method - Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132µL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 µg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14, 000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.
Background: BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-) . Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT3G01500
Category: RUBISCO/Carbon metabolism
Research Area: Plant Biology
