Euro
USD
British Pound
NULL
Product Description
BirA (mutated/TurboID) | AS20 4440 | Agrisera
Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177) . Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag) .
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: Collection of antibodies to various epitope Tags
Recommended Dilutions: 1 : 5000 (WB)
Molecular weight: 35 kDa
Confirmed Reactivity: BirA (mutated/TurboID)
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019) . Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: TAIR
Category: GAL4 / GUS / LUC / others
Research Area: Plant Biology
