Product Description
CCS | Chloroplastic copper chaperone for SOD | AS07 219 | Agrisera
Immunogen: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
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Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 33.8 | 37 kDa (Arabidopsis thaliana)
Confirmed Reactivity: Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays
Predicted Reactivity: Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Reconstitution: For reconstitution add 200 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT1G12520
Category: Heavy metal stress
Research Area: Plant Biology