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Product Description
GPX | Glutathione peroxidase (chloroplastic) | AS04 055 | Agrisera
Immunogen: thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: ELISA (ELISA), Western blot (WB)
Related Products: AS06 181 | Anti-glutatione reductase rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)
Molecular weight: 25 | 20 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms) . Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
Background: GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS) . Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT4G31870
Category: Oxidative stress
Research Area: Plant Biology
